OVER EXPRESSION OF ASPARTOKINASE GENE IN CORYNEBACTERIUM GLUTAMICUM FOR HIGHER PRODUCTION LEVELS OF L-LYSINE
The Identification of novel target genes to improve the lysine production is a big task for researchers. The present research work deals with the over expression of Aspartokinase gene in PBR322 plasmid present under a constitutive promoter. Aspartokinase upon overexpression in recombinant Corynebacterium glutamicum ATCC 13032 cells exhibited greater L-lysine production from 2.9 mg/mL by traditional method to 3.5mg/mL after 96 h of fermentation period. The study revealed that the optimum parameters were: temperature of 30°C, 7.5 pH, agitation speed of 200 rpm, 1 vvm air flow rate and glucose concentration of 90g/l to achieve maximum yield of L-lysine