133DEVELOPMENT AND VALIDTION OF UV SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF TOLPERISONE HYDROCHLORIDE AND PARACETAMOL FROM COMBINED TABLET DOSAGE FORMTwo simple, sensitive, rapid, accurate and economical methods were developed for the estimation of Paracetamol (PCM) and Tolperisone Hydrochloride (TOL) in their combined dosage forms. The first method is based on the simultaneous equation and second method is based on the First order derivative method. For the method I, the estimation of PCM and TOL were carried out in 243.2nm (λmax of PCM) and 261nm (λmax of TOL) respectively. For method II, estimation of PCM was carried out at 227nm (ZCP of TOL) and of TOL at 243.2nm (ZCP of PCM). The developed methods were validated in terms of linearity, accuracy, precision, sensitivity as per ICH Q2A guidelines. The developed methods could be routinely applied for quality control and analysis of PCM and TOL in their marketed formulation.umangshah.ph@ecchanga.ac.inResearch342012Paracetamol,Tolperisone hydrochloride,UV-visible spectrophotometer,Combined dosage forms,Method validationUmang Shah,Krupa Thula,Manan Raval,Pankti DesaiDepartment of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science & Technology, Changa-388421Anand, Gujarat,India.,Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science & Technology, Changa-388421Anand, Gujarat,India.,Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science & Technology, Changa-388421Anand, Gujarat,India.,Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science & Technology, Changa-388421Anand, Gujarat,India.