182METHOD DEVELOPMENT AND VALIDATION OF ATOMOXETINE HCl IN PURE AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHODA simple and reliable reverse phase high-performance liquid chromatography method was developed and validated for Atomoxetine HCl in pure and pharmaceutical dosage form. The method was developed on symmetric C18 (4.6 x 150mm, 5 m, Make: Agilent), with a mobile phase of phosphate buffer (PH 3.0): Methanol (30:70) %v/v. The effluent was monitored by Waters HPLC model containing Alliance 2695 with 2487 detector, variable wavelength prominence UV/ VIS detector SPD- 20A (VP series). Calibration curve was linear over the concentration range of 100 –140μg/ml. For inter–day and intra–day precision % relative standard deviation values were found to be 0.07% and 0.3% respectively. Recovery of Atomoxetine HCl was found to be in the range of 99.80 -101.40%. The limit of detection (LOD) and quantitation (LOQ) were 0.298 and 0.998μg/ml, respectively. The retention time and run time was very short; hence it is cost effective, making it more economical and rapid. Hence, this method can be used for the analysis of large number of samples.ramyarams803@gmail.comResearch372012Atomoxetine Hcl,RP-HPLC,Validation and Pharmaceutical dosage formRamya kota,P. Venkateswararao,A.M.S. Sudhakar babu,M. ArchanaDepartment of Pharmaceutical Chemistry, A.M.Reddy Memorial College of Pharmacy, Narasaraopet, Guntur district, Andhra Pradesh, India.,Department of Pharmaceutical Chemistry, A.M.Reddy Memorial College of Pharmacy, Narasaraopet, Guntur district, Andhra Pradesh, India.,Department of Pharmaceutics, A.M.Reddy Memorial College of Pharmacy, Narasaraopet, Guntur district, Andhra Pradesh, India.,Department of Pharmacy, K.L.E College of Pharmacy, Belgaum, Karnataka, India