20EVALUATION OF ANTIMUTAGENIC POTENTIAL OF ANNONA SQUAMOSA LEAF EXTRACTThe aim of the current study was to evaluate antimutagenic activity of ethanolic extract of Annona squamosa leaf which is belonging to the family Annonaceae. Its in vitro and in vivo antioxidant activity of the extracts has already been reported. On the basis of that we have decided to evaluate its antimutagenic activity in vivo in mice using bone marrow chromosomal aberration assay, micronucleus test and sperm abnormality assay. In chromosomal aberration assay, ethanolic extract of Annona squamosa (500mg/kg) had shown significant reduction in percentage of cells with aberration. In Micronucleus test, ethanolic extract of Annona squamosa (500mg/kg) showed significant reduction in total no. of micronucleus in polychromatic erythrocytes. In sperm abnormality assay, ethanolic extract of Annona squamosa (250 and 500mg/kg) had shown significant reduction in total no. of abnormality in sperm shape. In conclusion, these results suggest that ethanolic extract of Annona squamosa leaves possessing significant antimutagenic property.jay_143143@yahoo.comResearch122010Annona squamosa,Anticlastogenic activity, Mutagenicity,Chromosomal aberration assay,Micronuclease test,Sperm abnormality assay,Antioxidant activityThakkar Jalaram H,Solanki Himanshu K,Tripathi Pankaj, Patel Natvarlal J,Jani Girish KDepartment of pharmacy, SSR College of pharmacy, Sayli road, Silvassa, UT of D & NH, INDIA,Department of pharmacy, SSR College of pharmacy, Sayli road, Silvassa, UT of D & NH, INDIA,Department of Pharmacology, S.K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva, Mehsana-390 001, Gujarat, India,Department of Pharmacology, S.K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva, Mehsana-390 001, Gujarat, India,Department of pharmacy, SSR College of pharmacy, Sayli road, Silvassa, UT of D & NH, INDIA