223AGE DEPENDENT RELATIVE RISK OF ALUMINUM TOXICITY: LEVELS OF METALS AND ENZYMIC AND NON ENZYMIC ANTIOXIDANTS STATUS IN LIVER, KIDNEY AND BRAIN OF ALUMINUM TREATED YOUNG AND OLD RATSTo date, information on the interaction of Al with oxidative stress parameters in the rat organs in relation to age was limited and unclear. The purpose this study was to investigate the effects of Al-induced toxicity on age (young and old) and organs (brain, kidney and Liver) of male albino rats. Biomarkers are lipid peroxidase (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and oxidized glutathione (GSSG) along with metalsAl and Fe were investigated in young (6 months) and old (24 months) male rats following 100mg/kg body wt/day of aluminum chloride (AlC3) administration for 90 days. Results show that the oral administration of AlC3 promotes metals and oxidative stress in organs significantly (p<0.01) and the retention was high in old than the young. Brian of young and kidney of old was found to be the most sensitive whereas liver of young and brain of old the least. Al increases Fe, LPO, and GSSG and decreases SOD, CAT, GPx, GSH, and GSH/GSSG ratio, significantly (p<0.01) in comparison to control. No significant (p>0.05) difference were observed in the activity of CAT and GPx and level of LPO, GSH, GSSG and ratio of GSH/GSSG in the organs of young treated and old untreated rats suggests treatment made young equivalent to old. This study finds Al, Fe and GPx as valuable biomarkers of oxidative stress for organs in aluminum induced toxicity. These findings suggest that Al may produce pro-oxidant effect in rats and could be of interest for understanding the controversial role of Al in assessing toxicity.sandeeptripathiphd@gmail.comResearch432013Al toxicity,Oxidative stress biomarkers,metals,ANOVA,Cluster analysisDevesh Kumar Joshi,Manisha Choudhary,Sandeep Tripathi,Mahendra Pal Singh Negi,Abbas Ali MahdiDepartment of Advance Sciences, NIET, NIMS University, Jaipur India.,Department of Advance Sciences, NIET, NIMS University, Jaipur India.,Department of Advance Sciences, NIET, NIMS University, Jaipur India.,Biometry and statistics Division, Central Drug Research Institute, Lucknow-226001, India,Departments of Biochemistry, King George’s Medical University, Lucknow, India.