254DEVELOPMENT AND VALIDATION OF PALIPERIDONE IN BULK AND PHARMACEUTICAL FORMULATION BY RP-HPLCA simple, specific, accurate, and precise reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the estimation of Paliperidone in pharmaceutical dosage form. The chromatographic separation of Paliperidone was achieved on a Verian C-18 column with 250 x 4.6 mm i.d. and 5 μm particle size, using UV detection at 238nm. The optimized mobile phase was consisted of Acetonitrile: Methanol: KH2PO4 buffer pH 2.5 (30: 30 : 40 v/v). The flow rate was 1ml / min and effluents were monitored at 238 nm. Chromatogram showed the main peak at a retention time of 2.586 ± 0.02 min. The linearity was found in the concentration range of 10-60 (μg/ml). The Correlation coefficient was 0.9998. The limit of detection and limit of quantitation for estimation of Paliperidone was found to be 0.038 (μg/ml) and 0.116 (μg/ml) respectively. Recovery of Paliperidone was found to be in the range of 99.97-100.03 %. Proposed method was successfully applied for the quantitative determination of Paliperidone in pharmaceutical dosage form.ksirisha88@gmail.comResearch462013 ICH guidelines,Method validation,Paliperidone,RP-HPLCSireesha Kancharla,G.S. Pavan,Nagakanyaka Devi Paladugu,Bonthu Satyanarayana,Deepthi Poloju,Shaik Irfan PashaMax Institute of Pharmaceutical Sciences,Velugumatla, Khammam, Andhra Pradesh, India.,Max Institute of Pharmaceutical Sciences,Velugumatla, Khammam, Andhra Pradesh, India.,College of Pharmaceutical Sciences, AcharyaNagarjuna University, Guntur, Andhra Pradesh, India.,Max Institute of Pharmaceutical Sciences,Velugumatla, Khammam, Andhra Pradesh, India.,Max Institute of Pharmaceutical Sciences,Velugumatla, Khammam, Andhra Pradesh, India.,Max Institute of Pharmaceutical Sciences,Velugumatla, Khammam, Andhra Pradesh, India.