258BIOLOGICAL ACTIVTIES, HPLC AND NMR OF BLEPHARIS GLOMERANSThe methanolic seed extract of B.glomerans, showed Biological activities like in vitro antioxidant and cytotoxic activity. The crude extract was subjected to HPLC which consists of two compounds which were known, and structures were elucidated based on the 1H NMR spectrumkkmundla@yahoo.comResearch462013B. glomerans,Antioxidant,Cytotoxicity,HPLC,NMR spectrumKiran Kumar Mundla,B. SitaramAssociate Professor, Department of Chemistry, PVP Siddhartha Institute of Technology, Vijayawada, Andhra Pradesh, India.,Associate Professor, Department of Chemistry, PVP Siddhartha Institute of Technology, Vijayawada, Andhra Pradesh, India.