336DEVELOPMENT AND VALIDATION OF UV- SPECTROSCOPIC METHOD FOR EPERISONE HYDROCHLORIDE IN PURE AND TABLET DOSAGE FORMOne simple, precise, rapid and accurate UV Spectrophotometric method was developed for the estimation of Eperisone hydrochloride in bulk and in tablet formulation. Eperisone hydrochloride is a newer drug used in arthritis; it has been launched in the market recently. Eperisone hydrochloride dissolved in methanol followed by double distilled water and prepared 10 µg/ml solution scanned in the Double beam UV Spectrophotometer. From the spectra 258 nm was selected as an analyzing wavelength. Calibration curve was plotted by using concentration versus absorbance. From the calibration curve it was found that Eperisone hydrochloride obeys Beer’s law in the range of 5 - 25 µg/ ml, the Correlation coefficient value found to be 0.9997. Percentage amount of Eperisone hydrochloride raw material was found to be 100.45 ± 1.1431. The amount of Eperisone hydrochloride in formulation was found to be 100.57 ± 1.3149. The precision of the method was studied by making repeated analysis. The recovery studies were also carried out to ensure the accuracy of the method by adding known concentration of pure drug to a pre analyzed formulation. The average percentage recovery for formulation was found to be in the range of 100.80 to 102.80. The method was further studied by intraday and inter day analysis. The method showed excellent sensitivity, reproducibility, accuracy and repeatability, which is evidenced by low percentage relative standard deviation. The results obtained in recovery studies were indicating that there is no interference from the excipients used in the formulation. Hence it is suggested that the proposed UV spectrophotometric method can be effectively applied for the routine analysis of Eperisone hydrochloride in bulk and in tablet formulation.jothies_82@yahoo.co.inResearch4122013 Eperisone hydrochloride,Methanol,Double distilled water,UV Spectrophotometric MethodD.Jothieswari,S.Praveen Kumar,C.Hari Prasad,K.Prudhvi Raj,R.Ganesh,K.Bhaskar Reddy,K.Dhanalakshmi Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India, Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India, Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India, Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India, Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India, Sri Venkateswara College of Pharmacy, R.V.S. Nagar, Chittoor – 517 127, Andhra Pradesh, India,KLR Pharmacy College, Palvocha, Khammam Dist – 507 115, Andhra Pradesh, India