356EVALUATION OF HEPATO PROTECTIVE ACTIVITY OF ETHANOLIC ROOT EXTRACT OF TINOSPORA SINENSISThe present study was aimed to evaluate the protective effect of the ethanolic root extract of Tinospora sinensis on carbon tetrachloride induced hepatotoxicity. The extract was evaluated for hepatoprotective activity against ccl4-induced hepatotoxicity using rat liver. Hepatic enzymes studied include Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP). Hepatic injuries involved with possible necrosis which may have contributed to increase level of hepatic enzyme levels. Tinospora sinensis root extract was administered orally by dissolving in water. The root extract was screened for oral toxicity studies according to OECD guidelines 423. LD50 was calculated for selection of dose. Liver was excised and histopathological study was conducted by keeping in 10% formalin. They were stained with haematoxylin and eosin and photographed. Results showed that treatment with ethanolic root extract of Tinospora sinensis normalization of cells and reduced sinusoidal dilation along with mild inflammagens which are compared with Silymarin. This was evident from significant reduction in P<0.05, P<0.01, P<0.001 in serum enzyme levels. In the hepatoprotective and curative period, the highest damages in liver tissue were found in the order Carbon tetrachloride > low dose > high dose> silymarin> normal control. This clearly explained the reason for the Hepatoprotective activity of pomegranate root extract. It was concluded from the result that the ethanolic extract of Tinospora sinensis root possess hepatoprotective activityrameshmalothu@yahoo.co.inResearch4122013Carbon tetrachloride,Hepatoprotective activity,Histopathology,Tinospora sinensisNarendra Naik D,Salma Fathima S,Khandekar Durga,Ashwani K,Elumalai A,Ramesh MalothuDepartment of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological UniversityKakinada, Andhra Pradesh, India- 533003,Department of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological UniversityKakinada, Andhra Pradesh, India- 533003,Department of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological UniversityKakinada, Andhra Pradesh, India- 533003,Department of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological UniversityKakinada, Andhra Pradesh, India- 533003,Department of Pharmacognosy, SRM College of Pharmacy, Kattankulathur, Chennai, Tamilnadu, India,Department of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological UniversityKakinada, Andhra Pradesh, India- 533003