390HEPATOPROTECTIVE ACTIVITY OF BORRERIA HISPIDA L ROOTS AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ALBINO WISTAR RATSTo evaluate the Hepatoprotective activity of methanolic and ethyl acetate extracts of Borreria hispida L. roots against carbon tetrachloride induced hepatotoxicity in albino wistar rats. Animals were divided into nine different groups containing of six rats each. Group I served as normal control received distilled water (1ml/kg, p.o) daily for 7days and received olive oil (1ml/kg, s.c) on days 2 and 3. Group II served as CCl4 control and received distilled water (1ml/kg, p.o) daily for 7days. Group III was treated with the standard reference drug Silymarin (50mg/kg, p.o) daily for 7days. Animals of groups IV-VI were treated with ethyl acetate extract of Borreria hispida roots (EAEBH) at doses of 100mg/kg, 200mg/kg, and 300mg/kg respectively for 7days. Animals of groups VII-IX were treated with methanolic extract of Borreria hispida roots (MEBH) at doses of 100mg/kg, 200mg/kg, and 300mg/kg respectively for 7days. Groups II-IX received CCl4: olive oil (1:1, 2ml/kg, s.c) on days 8 and 9, 30min after administration of extract. After the course the treatment, the animals were fasted overnight and euthanized by decapitation under ether anaesthesia and blood was collected from jugular vein. Centrifugation at 2500rpm at 300C was done to separate the serum. The serum was subjected to various biochemical estimations. To study the liver function, the transaminase enzymes such as SGOT, SGPT and SALKP levels in the serum were essayed. To evaluate the action of Borreria hispida L., histopathological study was done to measure the characteristics such as centrizonal necrosis, sinusoidal dilation and hepatic fatty degeneration. Results revealed that, there is a tremendous elevation in the liver enzymes such as SGOT, SGPT and SALKP associated with CCl4 administration in Group II. In addition to that, administration of CCl4 in rats caused liver tissue abnormalities such as centrizonal necrosis, sinusoidal dilation and hepatic fatty degeneration. In groups IVVI, there is a significant decrease in SGOT, SGPT, SALKP and bilirubin levels when compared to group II rats. The activity of the extracts is found to be dose dependent. In this present study, it has shown that Borreria hispida L. demonstrate a strong Hepatoprotective activity against CCl4 induced hepatotoxicity in rats.sampath.venu9@gmail.comResearch4122013Borreria hispida L,Carbon Tetrachloride,Hepatoprotective,Transaminase enzymes,Histopathological studySampath Kumar GV,Sri Vidya VVL, Ch.SandhyaDepartment of Pharmacology, College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India., Department of Pathology, Great Eastern Medical School, Srikakulam, Andhra Pradesh, India.,Department of Pharmacology, College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.