428IN SILICO STUDY OF MYCOPLASMA PNEUMONIAE M129 HYPOTHETICAL PROTEINSMycoplasma pneumoniae is a human pathogen , lack cell wall , therefore it obligates for parasitic lifestyle . The genome was annotated and got about 252 (36.63%) hypothetical proteins (M. pneumoniae M129) according to NCBI/GenBank database. This study was an attempt to characterize these proteins using Bioinformatics tools available on the websites. It has been found that there are many lipoproteins belong to different families. There were 11 (4.36%) proteins involved in regulation and 16 (6.35 %) as transporters. Most of hypothetical proteins undergo phosphorylation at different residues (Threonine, Tyrosine and Serine). The bacteria get transmembrane proteins with different number of segments. Large number of proteins interacts with many of other proteins, few of them performed no interactions using STRING database, in addition large number of proteins are not included in the STRING database and these need to wait or they are artifacts resulted during sequencingzaheanasserk@gmail.comResearch532014Mycoplasma pneumoniae M129,Hypothetical proteins,Phosphoproteome,Lipoproteins,Transmembrane proteinsZahra M. Al-Khafaji,Zainib H. A. Al-Rikabi,Ali Imad Mohammad MonerInstitute of Genetic Engineering and Biotechnology for Postgraduate Studies / University of Baghdad, IRAQ,Institute of Genetic Engineering and Biotechnology for Postgraduate Studies / University of Baghdad, IRAQ,Institute of Genetic Engineering and Biotechnology for Postgraduate Studies / University of Baghdad, IRAQ