444ANTI-MICROBIAL INVESTIGATION AND PHYTOCHEMICAL ANALYSIS ON ORGANIC SOLVENT LEAF EXTRACTS OF CALOTROPIS GIGANTEAThe leaves extract of Calotropis gigantea were screened for its anti microbial and phytochemical activities. The solvents used for the leaves extraction were Acetone, Chloroform and Petroleum ether. The extract was tested against infectious diseases causing fungal pathogens such as Aspergillus niger, candida albicans Aspergillus fumigatus and bacterial pathogens such as Bacillus cereus, Proteus vulgaris, and Escherichia coli using the Agar well diffusion method. The Acetone extract of Calotropis gigantea showed more activity against fungus like Candida albicans zone of diameter 14.5mm, and bacteria like Escherichia coli zone of diameter 17.5mm, when compared to other solvent extracts. The results confirmed that presence of Antifungal and Antibacterial activity in the shade dried extract of Calotropis gigantea against the human pathogenic organismsgouri.kandukuri@gmail.comResearch542014Organic solvent,Leaf Extract,Phyto-chemical Analysis,Antimicrobial ActivityK Gouri Sankar,B Amaralinga Reddy,B Sathya Chaitanya,B Satish Kumar,Ch SivaiahDepartment Of Pharmacognosy, vagdevi College Of Pharmacy, Gurazala, Guntur Dt., Andhra pradesh, India-522415,Department Of Pharmacognosy, vagdevi College Of Pharmacy, Gurazala, Guntur Dt., Andhra pradesh, India-522415,Department Of Pharmacognosy, vagdevi College Of Pharmacy, Gurazala, Guntur Dt., Andhra pradesh, India-522415,Department Of Pharmacognosy, vagdevi College Of Pharmacy, Gurazala, Guntur Dt., Andhra pradesh, India-522415,Department Of Pharmacognosy, vagdevi College Of Pharmacy, Gurazala, Guntur Dt., Andhra pradesh, India-522415