476ISOLATION OPTIMIZATION AND PRODUCTION OF L-GLUTAMINASE FROM SOIL CONTAINING COLIFORM BACTERIAL-Glutaminase can be effectively used for the treatment of acute lymphoblastic leukemia. For pharmacological and clinical studies, microbial sources are best for the bulk production of L-glutaminase. A reported work is mainly focus on the production of L-glutaminase from coliform bacteria. Coliform bacteria were isolated from the soil samples which were collected from the various places of Guntur by using modified ISP-5 medium and multiple tubes lactose fermentation method. Out of the 10 isolates AS-3 strain was found to be the Citrobacter sp. and also involved in production of L-glutaminase. It was characterized by taxonomical and morphological studies. The effect of carbon and nitrogen sources on the production of Lglutaminase was investigated. Glucose at 1.5% and Yeast extract at 1.5% exhibited maximum L-glutaminase production. The other parameters such as pH, temperature and incubation period were optimized and set at 8, 37°C and 26 hr respectively.Lglutaminase has been partially purified with ammonium sulphate (20-40%) kanpharm283@gmail.comResearch562014L-Glutaminase,Coliform bacteria, Modified ISP medium,Ammonium sulphateS.Kannan, K.Gourisankar,S.Tirupati Rao,R. Narasimha Naik,Ch.Balasekhara Reddy Department of Pharmaceutical Biotechnology, Vagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh-522415, India,Department of Pharmacognosy, Vagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh-522415, India, Department of Pharmaceutical Biotechnology, Vagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh-522415, India,Department of Pharmacology, Vagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh-522415, India.,4 Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurazala, Guntur, Andhra Pradesh-522415, India