478BIOSYNTHESIS OF COPPER NANOPARTICLES USING OCIMUM SANCTUM LEAF EXTRACT AND ITS ANTIMICROBIAL PROPERTYIn recent developments and implementation of new technologies, Nanorevolution unfolds the role of plants in green synthesis of nanoparticles. In this study, the copper nanoparticles were synthesized from the leaf extracts of Ocimum sanctum and standardization of different parameters like metal ion concentration, reaction time, pH and ratio in different concentrations were done. The synthesized nanoparticles were characterized by using UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) which shows some reducing compounds such as eugenols, terpenoids and some aromatic compounds. Finally antimicrobial activities were analysed against different pathogensjchitra21@gmail.comResearch562014 Ocimum sanctum,Nanoparticles,UV,FTIR,Antibacterial activityAnuj Bhasker,Rajalakshmi A,Krithiga N,Gurupavithra S,Jayachitra ADept.of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India,Dept.of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India,Dept.of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India,Dept.of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India,Dept.of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India