471ANTIMICROBIAL ACTIVITY OF STEM BARK OF MEDICAGO SATIVA BY AGAR WELL DIFFUSION METHODThis paper reports the investigation results of anti-microbial activity of alfalfa stem bark extract against four bacterial strains namely Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureas, E.coli using agar well diffusion method. Inhibition zones were significantly different based on concentration of extract. The maximum inhibition was seen in 300μg/ml concentration of extract. First we prepared plant extracts using standard procedure and we have seen the anti-microbial activity by using minimal inhibitory concentration (MIC) and the maximum amount of inhibition was observed in gram positive bacterial strainsdrvijayakuchana@gmail.comResearch562014Agar well diffusion method,Anti-microbial activity,Medicago sativaVijaya Kuchana,Sunitha Sampathi,Pamu Sandhya,D. JagadeeshR.G.R. Siddhanthi College of Pharmacy, Secunderabad, Andhra Pradesh - 500 003, India,National Institute of Pharmaceutical Education and Research, Hyderabad, Andhra Pradesh - 500 037, India,Shadan Womens College of Pharmacy, Khairatabad, Hyderabad, Andhra Pradesh - 500 004, India,R.G.R. Siddhanthi College of Pharmacy, Secunderabad, Andhra Pradesh - 500 003, India