524BACTERIAL PROFILE AND DRUG SUSCEPTIBILITY PATTERN OF URINARY TRACT INFECTIONIntroduction: Urinary tract infection is most important cause of mortality and morbidity in this world affecting all age groups. Resistance to antibiotic is highly prevalent in bacterial isolates all over the world particularly in developing countries and is evolving and growing problem in UTI. Methodology: The study was conducted at ALKA Hospital, Lalitpur from February to November, 2013. Urine samples were collected and cultured; the culture positive isolates were subjected for antibiotic susceptibility testing by modified Kirby Bauer Method. Results: E. coli was found the most predominant organisms followed by Klebsiella pneumonia. Gram negative bacilli showed best susceptibility towards nitrofurantoin followed by gentamicin whereas ampicillin was found out to be the least effective drug. Cloxacillin was found to be the most effective followed by nitrofurantoin against gram positive bacteria. Multidrug resistance (MDR) was observed in 66.3% of total bacterial isolates. Conclusion: The antibiotic resistance among urinary isolates was high among gram negative bacteria than gram positive ones, E. coli being the most frequent. The highest susceptibility was shown by nitrofurantoin against gram negative isolates and by cloxacillin against gram positive isolates. Among MDR bacterial isolates, there are high resistance rates to almost all antimicrobial agentsrijalkomal@yahoo.comResearch5102014UTI,antibiotic,resistance,MDR,E. coliRajesh Kumar Sah,Komal Raj Rijal,Biswas Neupane, Binod Lekhak,Vijaya KumarDepartment of Microbiology, GoldenGate International College, Kathmandu, Nepal,Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal,Department of Microbiology, GoldenGate International College, Kathmandu, Nepal,Department of Microbiology, GoldenGate International College, Kathmandu, Nepal,Department of Microbiology, Alka Hospital, Lalitpur, Nepal