538ASSOCIATION OF LEPTIN 2549C/A GENE POLYMORPHISM WITH METABOLIC RISK MARKERS IN ADULT WOMEN OF NORTH INDIALeptin gene is one of potential candidate genes for metabolic syndrome. The present study was attempted to investigate whether the leptin gene polymorphism is associated with metabolic syndrome in Indian population. Totally 615 subjects were enrolled for the study, 305 women were with metabolic syndrome and 310 women were without metabolic syndrome according to NCEP-ATP III criteria. Anthropometric measurements were done in all the subjects. Fasting circulatory level of leptin, insulin, plasma glucose and lipid profiles were estimated along with calculation of insulin resistance. Leptin- 2549C/A promoter region polymorphism was done by RFLP method. Homozygous mutant genotype (AA) (CC v/s AA) (p=0.03: OR=1.68: 95% CI= 1.06- 2.68) and mutant allele (A) (p=0.02: OR=1.30: 95% CI= 1.04- 1.63) of the 2549 C/A leptin polymorphism were significantly observed in study population. The presence of the A allele were significantly associated with systolic blood pressure (p= 0.01), diastolic blood pressure (p=0.03), LDL (p=0.01), TC/HDL (p=0.03) and LDL/HDL (p=0.04) in study population. This study concludes that leptin 2549 C/A gene polymorphism was significantly associated with cardio metabolic risk in adult women of north Indiavaniresearch@rediffmail.comResearch5122014Leptin,polymorphism,Population,RFLP methodKumar Sandeep,Srivastava Nidhi, Mishra Supriya,Mishra Sameeksha,Natu Shankar Madhav,Roy Uma,Chandra Abhijit,Kumar Sandeep,Gupta VaniDepartment of Physiology, 2Department of Chemical Pathology,King George’s Medical University, Lucknow, India,Department of Physiology, 2Department of Chemical Pathology,King George’s Medical University, Lucknow, India,Department of Physiology, 2Department of Chemical Pathology,King George’s Medical University, Lucknow, India,Department of Physiology, 2Department of Chemical Pathology,King George’s Medical University, Lucknow, India,Department of Chemical Pathology, King George’s Medical University, Lucknow, India,Department of Biochemistry, Central Drug Research Institute, Lucknow, India.,Department of Gastroenterology, King George’s Medical University, Lucknow, India,All India Institute of Medical Sciences, Bhopal, India,Department of Physiology, 2Department of Chemical Pathology,King George’s Medical University, Lucknow, India