596UV-SPECTROPHOTOMETRIC DETERMINATION OF PIROXICAM IN BULK AND PHARMACEUTICAL DOSAGE FORM USING HYDROTROPIC SOLUBILIZATION TECHNIQUEHydrotropic solvents may proper choice to preclude the use of organic solvents so that, a simple, accurate, novel, safe and precise method could developed for estimation of poorly water soluble drug, Piroxicam. Solubility of piroxicam is increased by using 1M piperazine as a hydrotropic agent. Piroxicam showed the maximum absorbance at 354 nm in method A,350-360 nm in method B and 252 nm in method C. At these wavelengths, hydrotropic agent and other tablet excipients did not show any significant interference in the spectrophotometric assay. The developed methods were found to be linear in the range of 2.5-12.5 µg/ml with correlation coefficients (R) of 0.997, 0.996 and 0.994 respectively. The mean percent label claim of tablets of piroxicam in formulation estimated by the proposed methods was found to be 98.05-101 %. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical parameters were found to be good accordance with the prescribe values. As hydrotropic agent was used in the proposed methods, these methods were eco-friendly and it can be used in routine quantitative analysis of drug in bulk and dosage form in industriesmasthanamma.sk@gmail.comResearch642015Piroxicam,Piperazine,AUC,Hydrotropic solubilization technique,Derivative spectroscopySk.Masthanamma,T. Ananta Sridha,K.Nagaraju,T.Anil KumarDepartment of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjunanagar, Guntur–522510. Andhra Pradesh, India.,Department of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjunanagar, Guntur–522510. Andhra Pradesh, India.,Department of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjunanagar, Guntur–522510. Andhra Pradesh, India.,Department of Pharmaceutical Analysis, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjunanagar, Guntur–522510. Andhra Pradesh, India.