686AMELIORATIVE EFFECT OF OROXYLUM INDICUM [L.] VENT. METABOLITES ON TOBACCO EXTRACT INDUCED CELL DAMAGE IN HUMAN LYMPHOCYTESRoot of Oroxylum indicum (L.) Vent. has been used in different ayurvedic preparations like Chyavanprash and Dashmularisht. Its root extracts are rich in antioxidants and flavonoids, so they were tested for reversal of damage to lymphocytes caused by tobacco extract (TE). The aim of the study was to observe the effects of aqueous and n -butanol fractions of root extract of Oroxylum indicum when applied to lymphocytes in vitro to study their ameliorative effect on TE injured lymphocytes. Antioxidant activity of the root extract was determined by DPPH method . Both extracts were chemically analysed by GC-MS. The toxicity of various concentrations of TE was tested on lymphocytes which were exposed to TE for 2 hours. The ameliorative effects of aqueous and n-butanol fractions of root extracts of Oroxylum indicum were observed on the same cells. Every test was performed in triplicate and statistically analysed by Student’s t- test. It was observed that cell viability was significantly enhanced when TE injured cells were treated with aqueous extract. Both the extracts had a high occurrence of baicalein and chrysin. TE treatment on human lymphocytes in vitro was harmful, as the survival rate of lymphocytes decreased after TE exposure. Survival rate of damaged lymphocytes was enhanced after root extract treatment. The cell viability enhancement of lymphocytes after TE treatment is due to the activity of the antioxidants present in both extr acts. Out of two root fractions, aqueous fraction exhibited better ameliorative effectmamtashrirang@gmail.comResearch6112015Ameliorative effect,Human lymphocytes,Oroxylum indicum,Root extract,Tobacco extractMamta Gokhale,Asha Khanna,Dayashankar GautamDepartment of Biotechnology, St. Aloysius College (autonomous), Jabalpur, Madhya Pradesh, India,Department of Zoology, St. Aloysius College (autonomous), Jabalpur, Madhya Pradesh, India,Department of Zoology, St. Aloysius College (autonomous), Jabalpur, Madhya Pradesh, India