727TYROSINASE INHIBITORY ACTIVITY OF ISOLATED USNIC ACID FROM ROCELLA BELANGERIANA (LICHEN)The current investigation was carried out to determine the tyrosinase inhibitory activity of isolated usnic acid from Rocella belangeriana (lichen). The tyrosinase enzyme was obtained from crude mushroom and its solution was made using phosphate buffer of pH 6.8. Dimethyl sulphoxide (DMSO) was used for the preparation of usnic acid. A clear solution of dihydroxyphenyl alanine, phosphate buffer, test solution and crude enzyme solution was made and its absorbance was taken at 475 nm. In a same way control was made and its absorbance was taken at same 475nm. The percentage inhibition of tyrosinase enzyme caused by standard usnic acid and isolated usnic acid were calculated and was found that isolated usnic acid showed good percentage of inhibitionrkmolu103@gmail.comResearch722016Rocella belangeriana,Tyrosinase,Usnic acidRita Kalai Chakma,Prakash Yoganandam GAssistant Professor, Faculty of Pharmacy, Bharat Technology, Uluberia, Jadurberia, Banitabla, Howrah, West Bengal- 711316, India,Assistant Professor, College of Pharmacy, MTPG&RIHS, Pudeucherry-605006, Tamil nadu, India