73DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METOPROLOL AND TELMISARTAN IN TABLET DOSAGE FORMA simple, sensitive and feasible reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of metoprolol (MET) and telmisartan (TEL) in bilayer matrix tablet. Chromatographic separation was carried out using C8 column (250 mm × 4.6 mm, 5 µm particle size) with mobile phase composition of 10 mM KH2PO4 buffer (pH 6.8) and acetonitrile (60:40, v/v) at a flow rate of 1 mL min-1. The effluent was monitored using UV detection at 224 nm. Carbamazepine was used as internal standard. Retention times of MET, TEL and IS were 4.92, 3.81 and 6.61 min, respectively. The method was found to be linear over the concentration range of 0.05 to 30 µg mL-1 with correlation coefficient (R2) value greater than 0.99 for both the analytes. This proposed method was validated for according to ICH guidelines for tablet dosage form. Results of this study complied with the above mentioned guidelines. This method was successfully applied to the estimation of both the components simultaneously in pharmaceutical tablet formulation.proftkpal@gmail.comResearch322012Metoprolol,Telmisartan,HPLC,Tablet assayUtpal Nandi,Sujata Ghosh Dastidar ,Tapan Kumar PalProfessor, Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, India.,Professor, Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, India.,Professor, Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700 032, India.