742PHYTOCHEMICAL INVESTIGATION OF THE FLOWER EXTRACTS HYBANTHUS ENNEASPERMUS (L.) F. MUELLHerbs have always been the principal form of medicine in India and presently they are becoming popular throughout the world. There is a widespread belief that green medicines are healthier and more harmless or safer than synthetic ones. Th e present study is aimed to investigate the phytochemicals present in the flower extracts of Hybanthus enneaspermus. Preliminary phytochemical screening was done using 5 different solvents. The ethanol extract showed high number of secondary metabolites and it was followed by acetone, water, petroleum ether and chloroform extracts. Hence ethanol extract was selected for further quantification study. Total phenol, flavonoid, alkaloid, steroid, saponin, terpenoid and tannin were quantified. Tannin content was found to be high when compared to other secondary metabolitespriyamohan729@gmail.comResearch742016 Flower extracts,phytochemicals, secondary metabolites,HybanthusMohanapriya M,Jayanthi J,Ragunathan MG G. S. Gill Research Institute, Guru Nanak College, Chennai- 6000 042, Tamilnadu, India,Department of Advanced Zoology and Biotechnology, Guru Nanak College, Chennai- 6000 042, Tamilnadu, India.,Department of Advanced Zoology and Biotechnology, Guru Nanak College, Chennai- 6000 042, Tamilnadu, India.