759METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEUOUS DETERMINATION OF LIDOCAINE AND METHYL PARABEN IN LIDOCAINE AND EPINEPHRINE INJECTION BY UPLC TECHNIQUELidocaine Hydrochloride is used in intravenous regional aneasthesia and nerve blocks. Since the method for determination of Lidocaine and Methyl paraben in Lidocaine and Epinephrine injection by UPLC it is s not described in current Pharmacopoeias, the aim of this work was to develop and validate a precise, accurate and robust method. Seperation was achieved on a RP-18 Column (20mm*4.6, 1.8µ particle size), column temperature kept as a ambient. In the mobile phase preparation mixed 50mL of glacial acetic acid and 90mL of water. Adjusted the pH to 3.50 with 1N Sodium Hydroxide. 800mL of the above solution is mixed with 200mL of Acetonitrile, mixed well, filtered and degassed. The flow ra te was 1.5mL/minute and UV detection wavelength at 254 nm. The method was validated by following ICH guidelines and Validation parameters showed that method can be used as stability indicating for determination of Lidocaine and Methyl paraben in Lidocaine and Epinephrine injectionshanmugapandiyan@gmail.comResearch772016Lidocaine Hydrochloride,Methyl paraben,UPLC,Validation,Method developmentR. Hari krishna,P.ShanmugapandiyanResearch Scholar, PRIST University, Tanjore, Tamilnadu, India,Principal, Rao’s College of Pharmacy, Nellore, Andhra Pradesh, India