810SYNTHESIS OF CERTAIN AZETIDIN-2-ONE DERIVATIVES FOR ANTITUBERCULAR ACTIVITIES BY ALAMAR BLUE ASSAY METHODIsoniazid condensed with different derivatives of acetophenone to form hydrazones, using Vilsmeier-Haack reagent to form free aldehyde which on reacts with different free amide (R-NH2) group to form imines(C=N) which on react with Chloro acetyl chloride and Triethylamine to gives Azetidin-2-one derivatives. The structures of the all newly synthesized compounds have been established on the basis of their spectral data and elemental analysis. The selected compound was evaluated for anti-tuberculosis activity by using Alamar Blue Assay method.grvelsp@gmail.comResearch932018Azetidin-2-ones, Vilsmeier-Haack reagent, Anti-tubercular activity and Alamar Blue Assay method.G.Rathinavel,K.L.SenthilkumarG.Rathinavel,K.L.Senthilkumar